Ondokuz Mayıs Üniversitesi Bilgi Paketi - Ders Kataloğu

Description of Individual Course Units

Course Unit CodeCourse Unit TitleType of CourseYearSemesterECTS
VMK608 Molecular identification And Typing Methods 927001 1 2 6
Level of Course Unit
Second Cycle
Objectives of the Course
To give theoritical and practical information about Molecular identification and typing methods of bacteria
Name of Lecturer(s)
Doç.Dr. Arzu FINDIK
Learning Outcomes
  1. Students will be able to apply and also evaluate PCR based methods using for identification of the bacteria
  2. Students eill be able to applu and also evaluate protein- and PCR based methods using for typing of bacteria
Mode of Delivery
Formal Education
Prerequisites and co-requisities
None
Recommended Optional Programme Components
None
Recommended or Required Reading
1. Abigail, A., Salyers D., Whitt, D. (1994): Bacterial Pathogenesis: A Molecular Approach, ASM Press, Washington, D.C.2. Arda, M, (1995): Biyoteknoloji (Bazi Temel İlkeler). Kükem Derneği Bilimsel Yayınlar No:3, Ankara. 3. Çevik, A. M. (1994): PCR ve İnfeksiyon Hastalıklarda Kullanımı. Seminer, Ankara Hastanesi Klinik Mikrobiyoloji ve İnfeksiyon Hastalıkları Bölümü.4. Çiftci, A. (2003): Antimikrobiyel Direncin Genetik Metotlar ile Saptanması. Orlab On-Line Mikrobiyoloji Dergisi.1:22-28.5. Eisentein, I. B. (1990): New Molecular Techniques for Microbiai Epidemiology and The Diagnosis of Infectious Diseases. J.Inf.Dis, 161:595-602.6. Rodriguez, J. M. (1997): Detection Of Pathogens By Using The Polymerase Chain Reaction (PCR). Vet.J.,153:287-305.7. Xu, L., Larzul, D. (1991): The Polymerase Chain Reaction: Basic methodology And Applications. Comp.Immun.Microbiol.Infect.Dis., 14:209-221.
Planned Learning Activities and Teaching Methods
Language of Instruction
Turkish
Work Placement(s)
None
Course Contents
Gene and protein based identification and typing methods
Weekly Detailed Course Contents
Week Theoretical Practice Laboratory
1.Nucleic acids
2.Polymerase Chain Reaction (PCR)-1
3.Polymerase Chain Reaction (PCR)-2
4.Application and principles of PCR-1
5.Application and principles of PCR-2
6.Inverse PCR, In Situ PCR, Hot Start PCR
7.Multiplex PCR
8.Nested PCR
9.Reverse-tranktriptase PCR
10.Real-time PCR
11.Detection of animal pathogens by PCR
12.Definition and importance of Molecular typing
13.Conventional typing methods
14.Protein based typing methods
15.DNA based typing methods
16.PFGE
Assessment Methods and Criteria
Term (or Year) Learning ActivitiesQuantityWeight (%)
Midterm Examination1100
SUM100
End Of Term (or Year) Learning ActivitiesQuantityWeight (%)
Final Examination1100
SUM100
Term (or Year) Learning Activities40
End Of Term (or Year) Learning Activities60
SUM100
Workload Calculation
ActivitiesQuantityTime(hours)Total Workload(hours)
Midterm Examination122
Final Examination122
Attending Lectures16464
Practice16116
Laboratory16116
Discussion16116
Brain Storming16116
Performance16116
Homework428
SUM156