Ondokuz Mayıs Üniversitesi Bilgi Paketi - Ders Kataloğu

Description of Individual Course Units

Course Unit CodeCourse Unit TitleType of CourseYearSemesterECTS
VMK623 Antigen-Antibody Reactions 927001 1 1 6
Level of Course Unit
Second Cycle
Objectives of the Course
Having experience about the importance of immunological test methods and its practices. And practicing serological tests
Name of Lecturer(s)
Prof. Dr. Timur GÜLHAN
Learning Outcomes
  1. Helps diagnose infection directly / indirectly
  2. Allows monitoring of vaccinated immune status
  3. Allows to determine the effectiveness with protection tests
  4. Helps with seroepidemiological studies
Mode of Delivery
Formal Education
Prerequisites and co-requisities
Recommended Optional Programme Components
Recommended or Required Reading
1- Immunology: A short course. 5 th ed., 2003. Richard Coico, Geoffrey Sunshine, Eli Benjamini.
2- İmmünoloji. Serdar DİKER
3- Veterinary Immunology, An introduction, 5 th ed., Tizard IR., 1996.
4- Practical Immunology,Frank C. Hay Olwyn M.R. Westwood,Fourth Edition, 2002.
5- Methods in Molecular biology. ELISA Theory and Practice. Crowther J.R.
6- Enzyme Immunoassay. Application to diagnosis of bovine brucellosis. Nielsen K., Gall D., Kelly W., Henning D., Garcia M
Planned Learning Activities and Teaching Methods
Language of Instruction
Work Placement(s)
Course Contents
Serological tests and their applications, extraction of antigens and quantifying of protein concentration of interest
Weekly Detailed Course Contents
Week Theoretical Practice Laboratory
1.Clonal selection theory Separation of sera and plasm and storage conditions
2.Affinity, avidity, prozon, postzon and cross-reactions Agglutination test and its applications
3.Immunglobulins Precipitation test and its applications
4.Immunglobulins Haemagglutination test and its applications
5.Antigen antibody reactions CFT and its applications
6.Antigenic molecules and their extraction procedures Antigen preparation
7.Antigenic molecules and their extraction procedures Antigen preparation
8.Antigenic molecules and their extraction procedures Preparation of subunit antigens
9.Antigenicity detection in bacterial extracts protein concentration detection of some antigens
10.Protein concentration detection ELISA and its applications
11.Immunoenzymatic tests ELISA and its applications
12.Electrophoresis and its applications Westernblot and its applications
13.Exam Westernblot and its applications
14.Fluoresans tests Radioimmunoassays Protection tests FAT Serum neutralisation test
Assessment Methods and Criteria
Term (or Year) Learning ActivitiesQuantityWeight (%)
Midterm Examination160
Laboratory Examination130
End Of Term (or Year) Learning ActivitiesQuantityWeight (%)
Final Examination1100
Term (or Year) Learning Activities40
End Of Term (or Year) Learning Activities60
Workload Calculation
ActivitiesQuantityTime(hours)Total Workload(hours)
Midterm Examination111
Final Examination111
Attending Lectures14228
Individual Study for Mid term Examination3515
Individual Study for Final Examination3515
Laboratory Examination122