Ondokuz Mayıs Üniversitesi Bilgi Paketi - Ders Kataloğu

Description of Individual Course Units

Course Unit CodeCourse Unit TitleType of CourseYearSemesterECTS
MİK723 Diagnostic Molecular Microbiology Methods 927001 1 2 6
Level of Course Unit
Third Cycle
Objectives of the Course
Using Molecular Methods in Microbiology
Name of Lecturer(s)
Learning Outcomes
  1. Academic knowledge has about molecular methods
  2. Classify can molecular methods
  3. The basic principles of molecular methods know
  4. The optimization of molecular methods know
  5. The location of molecular methods in microbiological diagnosis know
Mode of Delivery
Formal Education
Prerequisites and co-requisities
None
Recommended Optional Programme Components
None
Recommended or Required Reading
-Reddy CA. Methods for General and Molecular Microbiology. ASM PRESS, 3. Baskı, Washington, 2007
-Murray, PR. Rosenthal KS. Pfaller MA. Tıbbi Mikrobiyoloji, Atlas, 1. Baskı, Ankara, 2010
Planned Learning Activities and Teaching Methods
Language of Instruction
Turkish
Work Placement(s)
None
Course Contents
Using Molecular Microbiological Methods in Diagnosis
Weekly Detailed Course Contents
Week Theoretical Practice Laboratory
1.Classification molecular methods
2.Isolation nucleic acids (DNA and RNA)
3.PCR
4.Real-time PCR
5.Restriction enzymes and reverse transcription
6.Agarose gel electrophoresis
7.Restriction Fragment Analysis (RFLP) and Pulse Field Gel Electrophoresis
8.FİSH method
9.Mutation and recombination
10.Mutation, deletion and Recombination Detection Methods
11.Sequence Analysis Methods
12.Phylogenetic Analysis Methods
13.Conjugation, transfection and transformation
14.Maldi Tof
Assessment Methods and Criteria
Term (or Year) Learning ActivitiesQuantityWeight (%)
Midterm Examination1100
SUM100
End Of Term (or Year) Learning ActivitiesQuantityWeight (%)
Final Examination1100
SUM100
Term (or Year) Learning Activities40
End Of Term (or Year) Learning Activities60
SUM100
Workload Calculation
ActivitiesQuantityTime(hours)Total Workload(hours)
Midterm Examination111
Final Examination111
Attending Lectures14114
Individual Study for Mid term Examination14570
Individual Study for Final Examination14570
SUM156